Press Release: Targeting a-glucosidase for Potential Diabetes Treatment

Press Release: Targeting a-glucosidase for Potential Diabetes Treatment

Reducing unintended secondary effects remains a major obstacle for researchers tasked with developing new treatments for chronic diseases. A major impediment to generating drugs without side-effects is that these compounds are not always specific to their biological targets. Lack of specificity can cause side-effects when the drug interacts with non-target molecules in a different biological pathway than the intended target. Published this month in Journal of Young Investigators, Kennedy et al. examine the binding of eight small molecules against α-glucosidase — a common drug target for noninsulin‐dependent diabetes mellitus (type II diabetes).

Structural Analysis of a New Saccharomyces cerevisiae α-glucosidase Homology Model and Identification of Potential Inhibitor Enzyme Docking Sites

Structural Analysis of a New Saccharomyces cerevisiae α-glucosidase Homology Model and Identification of Potential Inhibitor Enzyme Docking Sites

To study potential inhibitory molecules of Saccharomyces cerevisiae α-glucosidase, a new homology model based on the most recent crystal structure of Saccharomyces cerevisiae isomaltase (PDB: 3A47) was built. This newest model was used to dock five known natural α-glucosidase inhibitors to explore the putative allosteric drug binding pockets. Examination of the docking simulations and in silico mutagenesis revealed a potential druggable pocket for binding and a critical lysine residue making thermodynamically favorable binding with the inhibitors. In order to support the data analyzed from the docking simulation a series of inhibition assays were conducted.